Cardoso expressed gratitude to Robertson for honoring her late husband's memory through Scotland's qualification achievement.
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"The World Cup was one of those dreams, a dream that the two of you nurtured, side by side, with the same passion with which you took to the pitch," she wrote.
The letter described how Jota's presence remains tied to Robertson's upcoming World Cup campaign.
"When I heard your words and learnt what you felt on that day when Scotland qualified for the World Cup, after so many years of waiting, I realised that Diogo never truly left the pitch," Cardoso wrote.
Cardoso noted that Robertson carries the shared dream of both players onto the international stage.
"By achieving that moment and securing your place at the World Cup, you won't be going alone. You'll be taking his dream with you too.
And when you step on to the pitch, I know it won't just be you walking out.
Diogo will be with you in your thoughts, in your steps, in your heart," she wrote.
Cardoso closed her letter by thanking the Scotland captain for transforming their grief into a meaningful tribute. "So today, I want to thank you.
Thank you for not forgetting him. Thank you for taking him with you.
Thank you for turning the pain of loss into strength and into something so beautiful," she wrote.
In other team updates, England players participated in a recreational round of golf with major champion Brooks Koepka at their training base.
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Security officials also confirmed that a recent shooting incident in Kansas City occurred approximately five miles from England's secondary base camp while the squad was stationed in Florida.