United States forward Folarin Balogun will miss the round of 16 World Cup match against Belgium on Monday after receiving a straight red card in the 2-1 victory over Bosnia and Herzegovina.
The red card was issued in the 64th minute following a VAR review by referee Raphael Claus for a challenge on Tarik Muharemovic.
A US Soccer spokesperson confirmed to The Guardian that the mandatory one-game suspension cannot be challenged under tournament regulations.
Appeals are only possible if the FIFA disciplinary committee extends the ban beyond one match.
In that case, any appeal would only seek to reduce the additional penalty back to a single game, leaving the initial one-game ban unavoidable.
Balogun, the United States' leading scorer in the tournament, will watch the elimination match from the stadium stands rather than the team bench.
The suspension follows a precedent where FIFA upheld a one-game ban for Paraguay's Miguel Almiron but increased Qatar's Assim Madibo to five games for a severe tackle.