United States forward Folarin Balogun will miss the round of 16 World Cup match against Belgium on Monday after receiving a straight red card in the 2-1 victory over Bosnia and Herzegovina.
The red card was issued in the 64th minute following a VAR review by referee Raphael Claus for a challenge on Tarik Muharemovic.
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A US Soccer spokesperson confirmed to The Guardian that the mandatory one-game suspension cannot be challenged under tournament regulations.
Appeals are only possible if the FIFA disciplinary committee extends the ban beyond one match.
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In that case, any appeal would only seek to reduce the additional penalty back to a single game, leaving the initial one-game ban unavoidable.
Balogun, the United States' leading scorer in the tournament, will watch the elimination match from the stadium stands rather than the team bench.
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The suspension follows a precedent where FIFA upheld a one-game ban for Paraguay's Miguel Almiron but increased Qatar's Assim Madibo to five games for a severe tackle.